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Investigating Microbiome Capacity of Caspian Sea by Metagenomics Technique

Iranian Biological Resource center (IBRC) researchers acquired  for the first time to examine microbiome  capacity of southern part of Caspian sea by metagenomics technique in a study, using high-performance sequencing, the structure of microbial population of Caspian sea was determined and genome of its outstanding microbes reconstructed.

“in this project, samples were collected from 15,50 and 150 meters of aquatic column of southern part of Caspian Sea and 16s rRNA gene sequencing was examined,” Dr.Shahzade’ Fazeli, the head of IBRC, said in this regard.

“The obtained results has shown the similarity of general structure of microbial population among these three depths, although differentiate with that of microbial community in aquatic environment of the ocean and fresh water of temperate environments,” he added. “Assembled genomes indicate to the presence of microorganisms in groups; Actinobacteria, Alphaproteobacteria, Betaproteobacteria,  Gamanaproteobacteria, Bacteroidetes, Cyanobacteria, Euryarchaeota and Thaumarchaeota.”

“The results of studying genomes relating to different depths of Caspian Sea, shows significant differences at the lower taxonomic levels which can be affected by environmental conditions such as light or temperature,” Dr. Fazeli pointed out.

The more accurate description of related genomes to Thaumarchaeota and Actinobacteria phyla and Alphaproteobacteria class as taxonomic groups whose members phylogenetically respond to changes in salinity percentage of the environment, shows that the most isolated genomes from Caspian Sea belonging to unknown microbial groups and in some cases have closer relationship to either fresh water or marine groups. The phylogenetic pattern of Caspian Sea genomes shows the multiple and separate phylogenetic origins of which are related to similar groups in freshwater and marine lineage.

09:25 - 01/01/2017    /    Number : 97    /    Show Count : 147