SDS-PAGE and western blotting workshop:

Headlines:

 

  • Fundamentals of protein evaluation
  • Different methods of protein evaluation
  • Protein extraction
  • Introduction of western blotting
  • Preparation of acrylamide gel
  • Protein electrophoresis on SDS-PAGE
  • Transmission of proteins
  • Blocking
  • Primary antibody incubation
  • Secondary antibody incubation
  • Chemiluminescence reaction
  • Densitometer and bands evaluation