1) Protein electrophoresis and Western blotting
Western blotting, also known as immunoblotting or protein blotting, is a core technique in cell and molecular biology. In most basic terms, it is used to detect the presence of a specific protein in a complex mixture extracted from cells. The Western blotting procedure relies upon three key elements to accomplish this task:
• the separation of protein mixtures by size using gel electrophoresis;
• the efficient transfer of separated proteins to a solid support;
• the specific detection of a target protein by appropriately matched antibodies.
Once detected, the target protein will be visualized as a band on a blotting membrane, X-ray film. Since Western blotting is accomplished rapidly, using simple equipment and inexpensive reagents, it is one of the most common laboratory techniques. The results achieved are also easy to interpret, unique, and unambiguous. Therefore, it is routinely used on its own, or along with other immunoassays, in research and clinical settings.
Sample submission condition: cell lysate, plasma or sera will be accepted.
Response time: two or three weeks after submission
2) RFLP services
Restriction Fragment Length Polymorphism defines as any variation in DNA between individuals revealed by restriction enzymes which cut DNA into fragments of different lengths in consequence of such variations. It is used forensically and in the diagnosis of hereditary diseases.
Human and Animal Cell Back offers wide range of restriction enzymes in order to cut DNA into fragments.
Response time: two weeks after request
3) Consultation Services
The Human and Animal Cell Bank offer a range of consultation services includes:
• Microscope services
• Establishment of cell culture rooms
• Establishment of Cell banks
• Establishment of required techniques in cell banking