1) MTT Cytotoxicity Assay
MTT assay is a colorimetric assay for assessing cell viability. It is considered as a basis for numerous in vitro assays of a cell population’s response to external factors. The reduction of tetrazolium salts is now widely accepted as a reliable method to examine cell proliferation.
Sample submission condition: Cultured cells can be provided by the applicant or can be obtained from Human and Animal Cell Bank. Samples must be provided by the applicant in proper form (must not contain toxic solvent such as methanol, acetone, DMSO). If containing toxic solvent, dried samples are preferred.
Response time: two weeks after sample submission.

2) XTT Cytotoxicity Assay
XTT assay is a colorimetric assay for assessing cellular proliferation, viability, and cytotoxicity. It is considered as a basis for numerous in vitro assays of a cell population’s response to external factors. The reduction of tetrazolium salts is now widely accepted as a reliable method to examine cell proliferation.
XTT assay versus MTT assay:
•    In contrast to MTT, the cleavage product of XTT is soluble in water; therefore, a solubilization step is not required.
•    XTT assay has more accuracy and sensitivity
•    XTT assay is less time consuming than MTT assay
Sample submission condition: Cultured cells can be provided by the applicant or can be obtained from Human and Animal Cell Bank. Samples must be provided by the applicant in proper form (must not contain toxic solvent such as methanol, acetone, DMSO). If containing toxic solvent, dried samples are preferred.
Response time: two weeks after sample submission.

3) Commet assay

4) Apoptosis services
Flow cytometry provides a fast method to identify two cell populations including apoptotic and non-apoptotic cells.  Plasma membrane permeability is detected to determine cell viability and apoptosis. Flow cytometric analysis of apoptotic and non-apoptotic populations is performed at human and animal cell bank using AnnexinV-FITC and 7- Aminoactinomycin D.
Services ordering information: Based on custom request

5) Chromosomal Aberration

6) Micronucleus
Genotoxicity assessment is routinely performed at human and animal cell bank to distinguish potential genetic carcinogenesis and germ cell mutagenesis of different types of substances ranging from pharmaceuticals, industrial chemical, food additives, and cosmetics aiming at the protection of human and animal safety. The DNA damage resulted in genetic toxicity of materials can be in various forms including single- and double-strand breaks, loss of excision repair, cross-linking, point mutations, and structural and numerical chromosomal aberrations. The compromised integrity of the genetic pool can cause cancer. In vitro genotoxicity includes at least two or three test procedures, such as comet assay, chromosome aberrations and micronucleus assay. Department of human and animal cell bank at IBRC is pleased to prove these methods based on OECD guidelines which can be a promising start to create an effective development in executive standards.