1) Mycoplasma Contamination Testing
Mycoplasma contamination testing methods include direct solid agar microbial culture, DNA fluorescent staining with Hoechst 33258, and PCR method. Human and Animal Cell Bank offers these testing services upon request.
Sample submission condition: Cultured cells in T25 cell culture flask (without filter) should be filled with media and sealed appropriately with parafilm to avoid contamination. Cells should be cultured in antibiotic-free media for at least 2 passages before testing. In addition, the medium should not be replaced for 3 days before shipment.
Response time: one month after sample submission.
2) Fungal and bacterial contamination testing
Sample submission condition: For fungal and bacterial contamination testing, samples can be shipped frozen in cryovial or cultured (in flask or tube). The containers should be sealed with parafilm. Each sample should be placed in separate plastic bag to avoid contamination. Cells should be cultured in antibiotic-free media for at least 2 passages before testing. In addition, medium should not be replaced for 3 days before shipment
Response time: two week after sample submission.
3) Viral contamination detection
Sample submission condition: Viral contamination detection in cell culture is performed for EBV, HIV, HCV and HBV in Human and Animal Cell Bank. In order to assess the cells for all four viruses minimum amount of 10 million cells and for only one virus aliquot of 5 million cells is required. Cells should be cultured in antibiotic-free medium for at least two passages. For tissue samples minimum amount of 100 mg is required.
Response time: ten days after sample submission.
4) Species Identification of Cell Lines
One of the main issues in cell culture research is cross-contamination and misidentification of cell lines which mostly occurs due to personal errors in cell culture rooms. Therefore, cell line authentication seems to be critical in research. Human and animal cell bank applies multiplex PCR approach for cell line verification. Species identified based on this method includes rabbit, mouse, rat, cat, dog, sheep, goat, horse, cow, Rhesus monkey, African green monkey, pig, hamster, human, camel, donkey, chicken, and some insects.
Sample submission condition: Sample cells should be centrifuged and stored at -80 ̊ C and submitted on ice. The minimum amount of 2×106 cells is required.
Response time: two weeks after submission.
5) Authentication of human cell lines
Human and Animal Cell Bank offers authentication of human cell lines by STR DNA analysis.
Sample submission condition: For extracted DNA samples, the required amount of sample is 25 µl which should be delivered in labeled 0.5, 1.5, or 2 ml tubes. The tubes should be sealed properly with parafilm. For cultured cells, cells should be detached from the flask using cell scrapper (not trypsin). After centrifugation and discarding the supernatant, cell should be stored at -80 ̊C until the shipment. The minimum amount of cells needed for STR is 2×106 cells. For blood samples, aliquot of 3 ml should be provided in heparin containing tubes.
Response time: two weeks after sample submission