1) Distribution of cell lines on request
We offer a wide range of human and animal cell lines upon the request. Cells are cultured in T25 cell culture flasks (without filter). For delivery, the flask is filled with complete medium. The cap is sealed appropriately with parafilm to avoid contamination.
Delivery time: two weeks after sample submission

2) Characterization of Mesenchymal stem cells (MSCs)
Researchers require a dependable and cost-effective source for Mesenchymal Stem Cells (hMSCs). These cells can be differentiated into cartilage, bone, fat, muscle and even neural cells. Here, at human and animal cell bank, by specific antibodies we investigate CD45 and CD 29 markers using flowcytometry. Also, for differentiation potential, we use osteogenic and adipogenic induced media and for confirmation oil red and alizarin red staining is used.
Sample submission condition: cultured cells in T25 cell culture flask (without filter) should be filled with media and sealed appropriately with parafilm. If sending more than one sample, each sample should be placed in separate plastic bag to avoid contamination. Cells should be cultured in antibiotic-free media for at least 2 passages before testing. In addition, the medium should not be replaced for 3 days before shipment.
Response time: minimum one month after sample submission.

3) Characterization of new cell lines
In recent years, several new cell lines have been established which can be introduced to academic institutions and industry; however, these cells need to be verified and characterized before application. With this regard, Human and Animal Cell Bank offer characterization services upon request.
Sample submission condition: cultured cells in T25 cell culture flask (without filter) should be filled with media and sealed appropriately with parafilm. If sending more than one sample, each sample should be placed in separate plastic bag to avoid contamination. Cells should be cultured in antibiotic-free media for at least 2 passages before testing. In addition, the medium should not be replaced for 3 days before shipment.
Response time: two weeks after sample submission.

4) Establishment iPS cell line

5) Establishment of Mammals’ Fibroblast Cell Lines
Preservation of genetic resources from different species particularly endangered species is of high scientific importance. One of the most effective approaches is to establish somatic cell lines using cryopreservation technique to conserve animal genetic resources. Isolated and cryopreserved cells will provide experimental materials for investigation in cell biology, animal cloning and genetic engineering and also genomics and post-genomics researches.
Sample submission condition: For fibroblast cell line establishment, a skin tissue fragment of 5×5 mm is required. The tissue fragment can be provided by the applicant or can be obtained by Human and Animal Cell bank upon request. Samples should be placed in a sterile tube containing sterile buffer with antibiotics. Samples should be stored at 4-8 ̊C until shipment. The tube should be sealed with parafilm and labeled appropriately and transferred to Human and Animal Cell Bank within 48 h. ID card preparation, species identification and mycoplasma contamination tests will be offered upon request.
Response time: one month after sample submission.

6) Cell culture media and reagents
Cell culture requires high quality media and reagents. To ensure reproducible results, media must be pure, free of contaminants, and consistent. Human and Animal Cell Bank offers a wide range of general and specific high quality media, specific buffers, growth factors, and other reagents.
Delivery time: one week after submission.

7) Assay ready cells
Human and Animal Cell Bank offers a wide range of human and animal cell lines cultured in desired culture dishes upon request.
Delivery time: two weeks after sample submission.

8) Providing the growth curve of cell lines upon the request           
Sample submission condition: cultured cells in T25 cell culture flask (without filter) should be filled with media and sealed appropriately with parafilm. If sending more than one sample, each sample should be placed in separate plastic bag to avoid contamination.
Response time: two weeks after sample submission.
   
9) Cell Line Validation Testing
Application of contaminated cell lines in research will result in data that may not be reproducible or dependable. Several publications warning the research community about contamination and the journals are considering a policy of rejecting papers that use data that derived from non-validated cell lines. With this regard, Human and Animal Cell Bank offer cell line verification testing service.
Sample submission condition: cultured cells in T25 cell culture flask (without filter) should be filled with media and sealed appropriately with parafilm. If sending more than one sample, each sample should be placed in separate plastic bag to avoid contamination. Cells should be cultured in antibiotic-free media for at least 2 passages before testing. In addition, the medium should not be replaced for 3 days before shipment.
Response time: two weeks after sample submission

10) Cell Culture Supernatants Preparation
Cell culture supernatant has many applications in biomedical studies. This service would be beneficial for those scientists who do not have related facilities available.
Response time: two weeks after request.

11) Safe Deposits
Human and Animal Cell Bank offers safe deposit service for safekeeping of cell samples in vapor phase of liquid nitrogen tank. Cell identification card and authentication services will be provided upon request. 
Sample submission condition: cultured cells in T25 cell culture flask (without filter) should be filled with media and sealed appropriately with parafilm. If sending more than one sample, each sample should be placed in separate plastic bag to avoid contamination.

12) Fluorescence & phase-contrast imaging
Human and Animal Cell Bank offers live cells and samples imaging services using Fluorescence & phase-contrast and bioluminescence microscopy. We offer a variety of options to facilitate the image-based research needs of individual investigator.
Response time: one week after request.

13) Separation of mono nuclear cell from peripheral blood with ficholl
Cell separation is a tool, which is widely used in many strands of biological and biomedical research. A large variety of cell separation methods are currently commercially available which are predominantly based on adherence, antibody binding and density such as Ficholl based techniques. Most regenerative treatments and research based on cell separation are restricted to tissues such as blood and bone marrow. Therefore, separation of mono nuclear cells from peripheral blood can be performed with gradient centrifuge and Ficholl.
Sample submission condition: For establishment of immortalized LBL; 3-5 cc of sample should be collected in vacuum venoject tube containing heparin as anti-coagulant under sterile condition. Then labeled appropriately and transferred to human and animal cell bank within 12 to 24 hours in optimum condition after sampling. Samples should be stored at 4-8 ̊C or cold box until shipment if the transfer is more than 24 hours.
Response time: two days after submission