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Establishment and Preservation of Lymphoblastoid Cell Lines from Fresh and Frozen Whole Blood and Mononuclear Cells

Blood cells are well-known and interesting sources for genome investigations. The complete genomic DNA can simply be isolated from blood, which can be collected without risk to the donor. However, one of the main problems in obtaining genomic DNA from blood samples is the restricted amount of DNA. This obstacle can be avoided by establishing EBV-transformed B cell lines. Recent developments in population studies show that EBV immortalization is a gold standard method for long-term maintenance of high molecular weight DNA.

Different methods have been reported generation of LCLs with unlike success rates. The cell bank has used four different methods to generate these cells in regarding reduce the cost, time, and increasing the success rate. The groups in this study were including fresh whole blood, frozen whole blood, fresh peripheral blood mononuclear cells (PBMCs), and frozen PBMCs. The samples were followed by EBV transformation to generate LCLs. Result showed that the freezing procedures did not have any considerable effect on the establishment of lymphoblastoid cells. As well, whole fresh blood should be directly transformed when the volume of the blood sample is less than 0.5 ml. These established cells have been preserved in the human and animal cell bank of the Iranian Biological Resource Center (IBRC) and are available for researchers. The results and comparison of these methods published entitle of "Establishment and Preservation of Lymphoblastoid Cell Lines from Fresh and Frozen Whole Blood and Mononuclear Cells" in the journal "In Vitro Cellular & Developmental Biology - Animal".


09:32 - 19/07/2021    /    Number : 1344    /    Show Count : 50