1- Protein Electrophoresis Using SDS-PAGE and Western Blotting:
• Basics of protein evaluations:
Various methods of protein evaluation
Protein extraction
Determination of protein concentration
• An introduction to western blot
• Laboratory section:
Preparation of Acrylamide gel
Protein transfer
Blocking blot paper
Incubation of primary antibodies
Incubation of secondary antibodies
Chemiluminescence reaction
• Visualization in dark roomDensitometry and evaluation of bands
2- Workshop on Working Techniques with Laboratory Animals:
• Ethical considerations of working with laboratory animals
• An introduction to animal models in biomedical research
• Principles of preservation and breeding of laboratory animals
• Methods of proper holding of laboratory animals
• Methods of maintenance and restraining animals
• Various models of anesthesia and their applications
• Methods of taking bloods and injections
• Zoonosis disease
• Necropsy
• Euthanasia
• Gestation
3- Preliminary Practical Techniques of Working in Clinical Laboratory:
• An introduction to laboratory equipment
• Basics of blood taking laboratories (Theoretical and Practical)
• Coagulation tests
• Urine analysis
• Hematology tests
• Biochemical tests
• Microbial tests
• Serological test
4- Advanced Practical Techniques of Working in Clinical Laboratory:
Headlines of hormonology:
Training on molecular identification using PCR method, Sperm analysis, hormonology
Enzyme Immunology
Radio Immunoassay and fundamental of other Immunoassays
Headlines of sperm analysis:
Analysis with manual and device-based methods according to latest recommendations of WHO
Morphological assessments with staining methods
Headlines of Eliza:
Basic immunology
Quality control of samplers
Different methods of Eliza
Viral group tests
Thyroid group tests
Tumor marker group test
Tests of sexual hormone group
Eliza (Practical)
Prerequisite: Preliminary applied techniques of working in clinical laboratory
5- High Performance Liquid Chromatography (HPLC) Training Course with a Focus on Agilent Device
• Theory and basic concepts of HPLC
• Getting to know the different parts of a device
• How to work with the device
• How to choose the right column: precise installation of the column, liquid and solid stationary phases, equivalent stationary phases, limitation and advantages of columns, how to choose column according to dimensions and type of stationary phase, …
• How to choose the mobile phase
• How to choose proper detector: principles of function, applications and limitation
• How to calibrate the device
• Analysis of samples and calculation of concentration
• Analysis and calibration methods: internal and external standard methods and standard development
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