gRNA synthesis for gene silencing and editing in animal cells with CRISPR technology


  • The basic principle of CRISPR/Cas9 genome editing technology
  • Introducing of knocked out(KO)/in and gene editing methods in animal cells with CRISPR/Cas9 technology (DNA base and DNA free systems)
  • How we could make CRISPR/Cas9 vectors
  • Detection of on-target and off-target before and after genome editing with CRISPR/Cas9 technology
  • Approaches to reduce CRISPR off-target effects
  • Designing CRISPR gRNA with software
  • In vitro gRNA  Synthesis, purification and cutting special vector with spCas9

Registration fee: $150
2-days workshop
Date: ……

Lecturer: Seyed Hossein Mousavi (Plant Biotechnology)