Molecular analysis and cloning


  • Bacterial genomic DNA extraction
  • Qualification and quantitation of extracted gDNA
  • Bacterial gene PCR amplification
  • Agarose gel electrophoresis
  • Isolation and purification of cloning desired DNA fragment by gel extraction method
  • Ligation reaction designing and preparation
  • Competent cells preparation
  • Bacterial transformation
  • Screening and culturing of recombinant colony
  • Plasmid extraction
  • Enzymatic digestion assay
  • Conclusion of cloning results

Registration fee: $ 100
2-days workshop
Date: ……

Lecturers: Reza Azarbaijani (Plant Biotechnology)
                    Seyyed Hossein Mousavi (Plant Biotechnology)