1- Methods of Molecular Identification of Filamentous Fungi
• Getting to know the importance and principles of molecular identification in systematic studies of fungi
• Principles of fungal genome extraction methods
• Practical training on DNA extraction
• Theoretical and practical principles of PCR and electrophoresis
2- Principles of Isolation, Phenotypic Identification and Cultivation of Filamentous Fungi
• Principles of phenotypic identification of fungi using macromorphology and micromorphology
• Training fungi cultivation
• Purification of fungi using single spore
• Practical training on slide culture preparation, preparation of wet mount and staining fungi
• Training on preparation of spore suspension with a certain spore population
3- Methods for Short-Term and Long-Term Preservation of Filamentous Fungi
• Principles of short-term preservation of fungi up to several month in 4ºC
• Principles of long-term preservation of fungi for more than 1 year using cryopreservation method in -80 ºC
• Principles of quality control process before, after and during preservation of fungi
• Principles of reviving fungi after preservation
• Calculating the efficiency of the preservation process in maintaining the viability of cells
4- Training Course on Isolation and Purification of Photosynthesizers Including Diatoms, Cyanobacteria and Microalgae
• An introduction to microalgae
• Methods of preparing culture media for photosynthetic microalgae
• Methods of purification and preservation of microalgae
• An introduction to morphological identification of microalgae
5- Principles of DNA Extraction and PCR Protocols of Microalgae (Algae, Diatoms and Cyanobacteria)
• An introduction to microalgae and optimum methods of DNA extraction
• Principles of identification and PCR of microalgae
• PCR (Practical)
• Gel electrophoresis and PCR troubleshooting
6- Workshop on Hematococcus and Extraction of Astaxanthin
• An overview of Hematococcus morphology
• Preparing culture media and cultivation of Hematococcus microalgae
• An overview of methods of creating physiological changes in Hematococcus microalgae
• Preliminary introduction to principles of extracting astaxanthin and determination of pigment content using spectrophotometric method
7- An introduction to Dunaliella and its Cultivation in Laboratory Scale
• Morphology of Dunaliella
• Methods of cultivation in laboratory scale
8- An Introduction to Spirulina Cyanobacterium and Determination of its Phycocyanin Content
• An overview of Spirulina and its pigments
• Principles of extraction and methods of measurement
• Measurement of phycocyanins with spectrophotometric methods
9- Evaluation of Probiotic Products/Strains According to the National Standard 19459
• An introduction to probiotic microorganisms
• Isolation of acid lactic bacteria
• Examination of probiotic properties (all the items mentioned in national standard 19459)
Practical tests: catalase, haemolysis activity, acid resistance, bile resistance, resistance to gastric enzymes
10- Food Microbiology: Evaluation of Contaminating and Pathogenic Microorganisms in Food and Cosmetic Industries
• An introduction to contaminating microorganisms in food products
• Methods of search, identification and counting microorganisms
• Investigation of microbial contamination in meat, diary, confectionary and chocolate product samples
• Practical workshop on isolation of contaminating and pathogenic microorganisms from a food product sample and a diary sample, identification, counting microorganisms using colony count and MPN method with confirmation tests
11- Microbial Culture Collections: Methods for Long-Term Preservation of Microorganisms (Theory)
• The principles of developing microbial culture collections, racking and data storage systems
• The principles and different methods for preservation and quality control of microorganisms including bacteria, archaea and fungi
12- Microbial Culture Collections: Methods for Long-Term Preservation of Microorganisms (Theory and Practical):
• The principles of developing microbial culture collections, racking and data storage systems
• An introduction to different preservation methods (short-term and long-term)
• Long-term cryopreservation of microorganisms in deep-freezing temperatures: sample in practical section: bacterial strain
• Long-term preservation of microorganisms using Lyophilization/ Freeze-drying method: sample in practical section: bacterial strain
• Quality control of the stored strains: Evaluation of the storage method and recovery potential after storage
• Recovery of a lyophilized ampoule containing a microbial strain
13- Long-Term Cryopreservation of Bacteria in Deep-Freezing Temperatures (Theory and Practical):
• The principles of developing microbial culture collections, racking and data storage systems
• The principles of long-term preservation of microorganisms
• Long-term preservation of extremophile and non-extremophile bacteria in deep-freezing temperatures (Cryopreservation)
• Quality control of the stored strains: Evaluation of the storage method and recovery potential after storage
14- Long-Term Cryopreservation of Fungi (Theory and Practical)
• The principles of developing microbial culture collections, racking and data storage systems
• The principles of long-term preservation of microorganisms
• Long-term cryopreservation of molds and yeasts in deep-freezing temperatures: sample in practical section: filamentous fungus strain
• Quality control of the stored strains: Evaluation of the storage method and recovery potential after storage
15- Long-Term Preservation of Microorganisms Using Lyophilization/ Freeze-Drying Method (Theory and Practical)
• The principles of long-term preservation of microorganisms
• Long-term preservation of microorganisms using Lyophilization/ Freeze-drying method: sample in practical section: bacterial strain
• Quality control of the stored strains: Evaluation of the storage method and recovery potential after storage
• Recovery of a lyophilized ampoule containing a microbial strain
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